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rhil 17b  (R&D Systems)


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    Structured Review

    R&D Systems rhil 17b
    Rhil 17b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+17b/Recombinant+Human+IL-17B+(CHO)+Protein/pm38672111-160-23-24
    Average 92 stars, based on 2 article reviews
    rhil 17b - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    other:

    Article Title: SCF FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation
    Article Snippet: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems, #8129-IL-025/CF), rhIL-17C (R&D Systems, #9640-IL-025/CF), rhIL-17E (R&D Systems, #8134-IL-025/CF), or rhIL-17F (R&D Systems, #1335-INS-025/CF) for 2 hours.

    Isolation:

    Article Title: SCF FBXW11 Complex Targets Interleukin-17 Receptor A for Ubiquitin–Proteasome-Mediated Degradation
    Article Snippet: .. The FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/mL rhIL-17A (R&D Systems, Minneapolis, MN, USA, #7955-IL-025/CF), rhIL-17B (R&D Systems, Minneapolis, MN, USA, #8129-IL-025/CF), rhIL-17C (R&D Systems, Minneapolis, MN, USA, #9640-IL-025/CF), rhIL-17E (R&D Systems, Minneapolis, MN, USA, #8134-IL-025/CF), or rhIL-17F (R&D Systems, Minneapolis, MN, USA, #1335-INS-025/CF) for 2 h. According to the manufacturer’s instructions, total RNA was isolated using Trizol reagent (Ambion, Carlsbad, CA, USA, #15596018) and quantified using a Take3 microplate spectrometer (Agilent BioTeck, Synergy H1, Santa Clara, CA, USA). .. A quantity of 1000 ng of RNA was used to synthesize cDNA by reverse transcriptase (Takara, San Jose, CA, USA, #RR037A).

    Migration:

    Article Title: Human Bone Marrow–Derived MSCs Can Home to Orthotopic Breast Cancer Tumors and Promote Bone Metastasis
    Article Snippet: Migration was assessed according to the manufacturer's instructions (Neuroprobe or Cultrex) and fixing, dying, (Diff Stain Kit; IMEB), and counting (ImageJ; NIH) cells, manually or with fluorescence emission. .. For IL-17B migration assays, 20 ng/mL of rhIL-17B (R&D Systems) was added to BCCs before seeding. ..



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    R&D Systems rhil 17b
    Rhil 17b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+17b/Recombinant+Human+IL-17B+(CHO)+Protein/pm38672111-160-23-24
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    R&D Systems rhil-17b
    <t>IL-17B</t> stimulation of IL-17BR can drive migration and metastasis. (A) Expression of il-17br was analyzed using qRT-PCR. Metastatic cells were compared with matched primary tumors. N=3 except for the SUM1315-BP2 mouse bone data point where too few metastases inhibited significance. (B) Serum-starved SUM1315, SUM1315-BP2 and MDA-MB-231 BCCs showed a dose-dependent reaction <t>to</t> <t>IL-17B</t> stimulation after 6-hours. No increase in migration was observed for MCF7 BCCs. (C) Serum-starved SUM1315 and MDA-MB-231 BCCs (mock infected or infected with lentivirus to overexpress il-17br cDNA) migrated for 6-hours on transwell migration assays. (D) Bioluminescent imaging of human bone fragments, lungs, and livers was used to assess metastasis frequency.
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    Image Search Results


    IL-17B stimulation of IL-17BR can drive migration and metastasis. (A) Expression of il-17br was analyzed using qRT-PCR. Metastatic cells were compared with matched primary tumors. N=3 except for the SUM1315-BP2 mouse bone data point where too few metastases inhibited significance. (B) Serum-starved SUM1315, SUM1315-BP2 and MDA-MB-231 BCCs showed a dose-dependent reaction to IL-17B stimulation after 6-hours. No increase in migration was observed for MCF7 BCCs. (C) Serum-starved SUM1315 and MDA-MB-231 BCCs (mock infected or infected with lentivirus to overexpress il-17br cDNA) migrated for 6-hours on transwell migration assays. (D) Bioluminescent imaging of human bone fragments, lungs, and livers was used to assess metastasis frequency.

    Journal:

    Article Title: Human bone marrow-derived MSCs can home to orthotopic breast cancer tumors and promote bone metastasis

    doi: 10.1158/0008-5472.CAN-10-1254

    Figure Lengend Snippet: IL-17B stimulation of IL-17BR can drive migration and metastasis. (A) Expression of il-17br was analyzed using qRT-PCR. Metastatic cells were compared with matched primary tumors. N=3 except for the SUM1315-BP2 mouse bone data point where too few metastases inhibited significance. (B) Serum-starved SUM1315, SUM1315-BP2 and MDA-MB-231 BCCs showed a dose-dependent reaction to IL-17B stimulation after 6-hours. No increase in migration was observed for MCF7 BCCs. (C) Serum-starved SUM1315 and MDA-MB-231 BCCs (mock infected or infected with lentivirus to overexpress il-17br cDNA) migrated for 6-hours on transwell migration assays. (D) Bioluminescent imaging of human bone fragments, lungs, and livers was used to assess metastasis frequency.

    Article Snippet: For IL-17B migration assays, 20 ng/mL rhIL-17B (R&D Systems) was added to BCCs before seeding.

    Techniques: Migration, Expressing, Quantitative RT-PCR, Infection, Imaging